Proteomics technologies are used for early detection and diagnosis of cancers for the development of novel therapeutic agents. Identification of biomarker and also the study of protein expression of the cancer are studied through proteomics platforms. These methods represent a heterogeneous group of techniques that can assess protein–protein interactions, protein modifications and tissue profiling. The Clinical Proteomic Technologies for Cancer and the Early Detection Research Network are efforts to identify protein signatures specific to different types of cancers. The protein arrays have in common the placement of a small amount of target material on a solid support, and they use a variety of techniques to identify enzymatic or protein-binding activity. Most of the proteins sug-gested in the literature as clinically useful molecules are still awaiting proper validation. According to American Medical Association (AMA) and Office of Cancer Clinical Proteomics Research at National Cancer Institute (NCI), the term of proteome was taken from two words; protein and genome, so prote- was taken from protein and -ome from genome. Hypothesis-testing is one of the most critical aspects of the cancer research. Cancer Proteomics. PTKs are involved in cell growth signaling. A proteomics research specialist in developing targeted mass spectrometry methods for quantifying proteins important in cancer Whiteaker, Jeff Fred Hutchinson Cancer Research Center, Seattle, WA, … They said that testing cancers for the presence of this protein may help clinicians identify patients who may be resistant to or relapse when treated with the therapy. Proteomics in Cancer Research Asian Pacific Journal of Cancer Prevention, Vol 6, 2005 115 protein purification in cancer research. So far, proteomics methods have revealed thousands of potential cancer biomarkers. Protein Visualization: Proteins are commonly visualized in gels by staining them with dyes such as colloidal Coomassie brilliant blue (CBB) G, silver stain and fluorescent stain (Gygi et al., 2000). The absence of a protein that works to prevent tumor formation may explain why some patients are resistant to a common cancer therapy, according to Penn State Cancer Institute and Penn State College of Medicine researchers. Tyrosine kinases (PTK) represent only 10% of all protein kinases, although they are the most important protein kinases. The Biomedical Proteomics Program is designed to identify protein signatures and design effective therapies for cancer patients. Main difficulties concern both complexity and heterogeneity of ovarian cancer and drawbacks of the mass spectrometry strategies. In order to understand proteomics, understanding what is proteome is necessary. 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